Additional Rapid Adapter and Adapter Buffer to enable customers to maximise the use of Rapid Barcoding Kits 24 and 96 V14 (SQK-RBK114.24 and SQK-RBK114.96).

This is an Early Access product

Note: This is only compatible with V14 chemistry

Additional library preparation reagents for the Native Barcoding Kit 24 and 96 V14 (SQK-NBD114.24 and SQK-NBD114.96).

This is an Early Access product

Note: This is only compatible with V14 chemistry

RNA Control Sample to support control runs for cDNA kits.

This is an Early Access product

Additional flow cell priming reagents for our V14 kits.

This is an Early Access product

Note: This is only compatible with V14 chemistry

The Sequencing Auxiliary Vials contain Sequencing Buffer (SB), Elution Buffer (EB), Library Solution (LIS) and Library Beads (LIB) with V14 flow cell priming reagents: Flow Cell Flush (FCF) and Flow Cell Tether (FCT).

This is an Early Access product

Note: This is only compatible with V14 chemistry.

An overnight method to prepare ultra-high molecular weight DNA for sequencing ultra-long reads (N50 >50 kb).

This is an Early Access product

This uses our latest Kit 14 chemistry

• Preparation time: ~200 minutes + overnight elution
• Read length: N50 >50 kb
• Input amount: 6 million cells

Simple and rapid library preparation, with barcoding for up to 96 gDNA samples.

This is an Early Access product

This uses our latest Kit 14 chemistry

• Preparation time: 60 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 50 ng dsDNA per sample

Simple and rapid library preparation, with barcoding for up to 24 gDNA samples.

This is an Early Access product

This uses our latest Kit 14 chemistry

• Preparation time: 60 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 50 ng dsDNA per sample

A simple and rapid library preparation method for genomic DNA optimised for speed with improved modal raw read accuracy.

This is an Early Access product

This uses our latest Kit 14 chemistry

• Preparation time: 10 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 100 ng dsDNA

A versatile method of preparing barcoded sequencing libraries optimised for median raw read accuracy of Q20+ (99%+) and long read multiplexed samples.

This is an Early Access product

Product lead time: 1 week

This uses our latest Kit 14 chemistry

• Preparation time: 140 minutes
• Read length: = to fragment length
• Input amount: 400 ng per sample of gDNA, 200 fmol per sample (130 ng for 1 kb amplicons)

A versatile method of preparing barcoded sequencing libraries optimised for median raw read accuracy of Q20+ (99%+) and long read multiplexed samples.

This is an Early Access product

Product lead time: 1 week

This uses our latest Kit 14 chemistry

• Preparation time: 140 minutes
• Read length: = fragment length
• Input amount: 400-1000 ng for gDNA. 200 fmol (130 ng for 1 kb amplicons) for amplicon input

A versatile sequencing kit optimised for median raw read accuracy of Q20+ (99%+) with long read singleplex samples. Multiple samples can be processed simultaneously.

This is an Early Access product

This uses our latest Kit 14 chemistry

• Preparation time: 120 minutes
• Read length: = fragment length
• Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR, 100-200 fmol of  amplicons or cDNA

A versatile sequencing kit optimised for median raw read accuracy of Q20+ (99%+) and long read singleplex samples.

This is an Early Access product

This uses our latest Kit 14 chemistry

• Preparation time: 60 minutes
• Read length: = fragment length
• Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR,100-200 fmol of  amplicons or cDNA

An expansion pack designed for size selection of high molecular weight DNA samples to progressively deplete short fragments of <25 kb.

Identification and quantification of full-length transcripts with highest output, with barcodes for up to 24 samples.

This uses our Kit 11 chemistry

This is an Early Access product

• Preparation time: 210 minutes + PCR
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 4 ng poly(A)+ RNA, or 200 ng total RNA per sample

The Sequencing Auxiliary Vials contain Sequencing Buffer II (SBII), Elution Buffer (EB), Loading Solution (LS) and Loading Beads II (LBII).

This expansion is suitable for use with Kit 10, Kit 11 and Kit 12 chemistry.

We do NOT recommend the Sequencing Auxiliary Vials expansion (EXP-AUX002) to be used with Kit 9 chemistry.

A sequencing kit optimised for output and PCR-free sequencing of 2-3 samples for Whole Genome Sequencing (WGS).

This is an Early Access product

This uses our Kit 11 chemistry

• Preparation time: 135 minutes
• Read length: = Fragment length
• Input amount: 1000 ng dsDNA per sample

The Flow Cell Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells on a higher scale.

This is compatible with all sequencing kits.

Identification and quantification of full-length transcripts with highest output.

This uses our Kit 11 chemistry

• Preparation time: 210 minutes + PCR
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 4 ng poly(A)+ RNA, or 200 ng total RNA

A simple, scalable and rapid method for sequencing up to 9,216 SARS-CoV-2 samples.

Product lead time: 3 weeks

A simple, scalable and rapid method for sequencing up to 2,304 SARS-CoV-2 samples.

Product lead time: 3 weeks

A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples.

Product lead time: 3 weeks

An expansion kit containing all the required reagents and primers to generate reverse-transcribed and PCR-amplified material from SARS-CoV-2 RNA samples.

Product lead time: 1 week

Note: This is only compatible with Kit 10 chemistry

• Preparation time: 80 minutes + PCR

Vials of Adapter Mix II

Note: This is only compatible with Kit 9 chemistry.

A versatile sequencing kit optimised for output, and long reads, and processing singleplex samples. We recommend using our latest version: Ligation Sequencing Kit V14 (SQK-LSK114).

This uses Kit 10 chemistry

• Preparation time: 60 minutes
• Read length: = fragment length
• Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR

The Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells.

This is compatible with all sequencing kits.

A ligation-based sequencing kit for processing singleplex samples. We recommend using our latest version: Ligation Sequencing Kit V14 (SQK-LSK114). All COVID-related projects will be supported indefinitely.

This uses Kit 9 chemistry

Includes a Flow Cell Priming Kit

This is a Legacy product

• Preparation time: 60 minutes
• Read length: = fragment length
• Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR

A simple and rapid library preparation method for genomic DNA. We recommend using our latest version: Rapid Sequencing Kit V14 (SQK-RAD114).

This uses Kit 9 chemistry

Includes a Flow Cell Priming Kit

• Preparation time: 10 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng dsDNA

This kit prepares genomic DNA libraries from as little as 100 ng DNA, using PCR.

This uses Kit 9 chemistry

Includes a Flow Cell Priming Kit

• Preparation time: 60 minutes + PCR
• Read length: = fragment length post-PCR
• Input amount: 100 ng dsDNA

Rapid and simple method of preparing up to 12 barcoded gDNA samples for sequencing from 1-5 ng using PCR.

This uses Kit 9 chemistry

Includes a Flow Cell Priming Kit

• Preparation time: 15 minutes + PCR
• Read length: ~2 kb
• Input amount: 1-5 ng dsDNA per sample

Ultra-Long DNA Sequencing Kit. We recommend using our latest version: Ultra-Long DNA Sequencing Kit V14 (SQK-ULK114).

This uses Kit 10 chemistry.

• Preparation time: 610 minutes
• Read length: N50 >50 kb
• Input amount: 6 million cells

This kit prepares up to 12 barcoded gDNA libraries from as little as 100 ng DNA, using PCR.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit

• Preparation time: 60 minutes + PCR
• Read length: = fragment length post-PCR
• Input amount: 100 ng dsDNA per sample

Simple and rapid library preparation, with barcoding for up to 96 gDNA samples. We recommend using our most recent version: Rapid Barcoding Kit 96 V14 (SQK-RBK114.96).

This uses Kit 10 chemistry.

• Preparation time: 60 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 50 ng dsDNA per sample

A simple and rapid library preparation method, with barcoding for up to 12 genomic DNA samples. We recommend using our most recent version: Rapid Barcoding Kit 24 V14 (SQK-RBK114.24).

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit

• Preparation time: 10 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng dsDNA per sample

Identification and quantification of full-length transcripts without PCR bias.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit

• Preparation time: 275 minutes
• Read length: Enriched for full-length cDNA
• Input amount: 100 ng poly(A)+ RNA

Prepare RNA for direct RNA nanopore sequencing.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit

• Preparation time: 115 minutes
• Read length: = RNA length
• Input amount: 500 ng total RNA, 50 ng poly(A)+ RNA

Cold chain-free library preparation for ultimate portability.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit

• Preparation time: 10 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng dsDNA

Genus-level bacterial identification with barcoding for up to 24 samples.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit

• Preparation time: 10 minutes + PCR
• Read length: = full-length 16S gene (~1.5kb)
• Input amount: 10 ng dsDNA per sample

A versatile sequencing kit optimised for throughput, long reads, and processing multiple samples simultaneously.

• Preparation time: 130 minutes
• Read length: = fragment length
• Input amount: 1000 ng dsDNA, 100+ ng DNA if performing fragmentation or PCR

Targeted sequencing of genomic regions using CRISPR-Cas9.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit

• Preparation time: 110 minutes
• Read length: = fragment length
• Input amount: 1–10 µg of dsDNA

This kit allows the user to carry out rapid 16S sequencing for bacterial identification on up to 12 barcoded samples at a time.

This uses Kit 9 chemistry.

Includes a Flow Cell Priming Kit

• Preparation time: 10 minutes + PCR
• Read length: = full-length 16S gene (~1.5kb)
• Input amount: 10 ng dsDNA per sample

Lambda DNA (LMD). Pack of six vials

PCR Primers (PRM) and PCR Adapters (PCA)

Rapid Sequencing Adapter (RAP) expansion pack.

Includes a Flow Cell Priming Kit

Please note: This is only compatible with Kit 9 chemistry.

The PCR Barcoding Expansion 1-96 (EXP-PBC096) is designed to allow the pooling and running together of multiple sequencing libraries.

• Preparation time: 150 minutes
• Read length: = to fragment length post PCR
• Input amount: 1000 ng dsDNA

The PCR Barcoding Expansion 1-12 (EXP-PBC001) allows the pooling and running together of multiple sequencing libraries.

• Preparation time: 150 minutes
• Read length: = to fragment length post PCR
• Input amount: 1000 ng dsDNA

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required. We recommend using our latest version: Native Barcoding Kit 24 V14 (SQK-NBD114.24).

Note: This is only compatible with Kit 9 chemistry.

• Preparation time: 80 minutes
• Read length: = to fragment length
• Input amount: 1000 ng dsDNA per sample

A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required. We recommend using our latest version: Native Barcoding Kit 24 V14 (SQK-NBD114.24).

Note: This is only compatible with Kit 9 chemistry.

• Preparation time: 80 minutes
• Read length: = to fragment length
• Input amount: 1000 ng dsDNA per sample

Short Fragment Buffer (SFB)

Note: This is compatible with all kit chemistries.

Flow Cell Priming Kit XL.

Please note: This is only compatible with Kit 9, 10, 11 and 12 chemistry.

The Sequencing Auxiliary Vials contain Sequencing Buffer (SQB), Elution Buffer (EB) and Loading Beads (LB), sufficient for 12 MinION/GridION or PromethION flow cells.

Please note: This is only compatible with Kit 9 chemistry.

Flow Cell Priming Kit.

Please note: This is only compatible with Kit 9, 10, 11 and 12 chemistry.

A versatile method of preparing a high number of barcoded sequencing libraries for applications where high throughput is required. We recommend using our latest version: Native Barcoding Kit 96 V14 (SQK-NBD114.96).

Note: This is only compatible with Kit 9 chemistry.

• Preparation time: 135 minutes
• Read length: = to fragment length
• Input amount: 400 ng dsDNA per sample, 200 fmol (130 ng for 1 kb amplicons) DNA per sample