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London Calling 2023
  17th - 19th May
VolTRAX Sequencing Kit
VSK-VSK004

A simple and rapid library preparation method for genomic DNA on the VolTRAX V2b device.

$140.00
2Early AccessAccess the very latest products and give feed back into the development process, subject to rapid upgrade. Warranty ~1 month, product lead time subject to availability. Products are fully tested and supported.

Information

This kit is recommended for users who:

  • Require a short preparation time
  • Would like to do automated library preparation on the VolTRAX V2b device

The VolTRAX Sequencing Kit (VSK-VSK004) is an upgrade to the VSK-VSK003 and generates sequencing libraries from extracted gDNA in 5-10 minutes hands-on time plus 45 minutes automation. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Sequencing Adapters are then added to the tagged ends, and the library is cleaned up using AMPure XP Beads.

The kit is optimised for simplicity, reducing hands-on time, and increased throughput. Due to the simple nature of the workflow and the precision of fluid handling on the VolTRAX V2b platform, the consistency in performance of libraries generated with this kit is greater than with other kits, and is less affected by user familiarity with library preparation.

VolTRAX Sequencing Kit features:

FeatureProperty
Preparation timeHands on time: 5-10 mins
Automation: 45 mins
Input requirement475 ng extracted DNA
PCR requiredNo
FragmentationTransposase-based
Read lengthRandom distribution, dependent on input fragment length
Read type produced1D
Associated protocolsVolTRAX V2b protocol
Pack size3 reactions
StabilityShipped at 2-8°C
Long-term storage -20°C, unopened, for 3 months

Shipping and logistics:

Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.

Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.

The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.

Protocols

VolTRAX protocol

To access the protocol, you will need to register for a Nanopore Community account.

What's in the box

The VolTRAX Sequencing Kit contains sufficient reagents to generate three sequencing libraries.

VSK-VSK004

NameAcronymCap colourNumber of vialsFill volume (µl)
VX Fragmentation MixVFAAmber125
EDTAEDTAClear120
VX AMPure XP BeadsVXPPink130
VX Long Fragment BufferVLFOrange140
VX Rapid Adapter TVRA TGreen125
VX Elution BufferVEBBlack165
VX Running BufferVRBRed1250
Flush BufferFBBlue31,170
Flush TetherFLTWhite cap, purple label1200

Compatibility

The VolTRAX Sequencing Kit can be used together with:

Kits

  • Flow Cell Wash Kit (EXP-WSH004)

Flow cells

  • FLO-MIN106D

Devices

  • VolTRAX V2b

The library prepared on the VolTRAX can be sequenced on:

  • MinION Mk1B
  • MinION Mk1C
  • GridION

Cartridge

  • VCT-V2002B